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Microglia pp 103-111 | Cite as

Analysis of Microglial Production of Reactive Oxygen and Nitrogen Species

  • Urte Neniskyte
  • Guy C. Brown
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1041)

Abstract

Reactive oxygen and nitrogen species are both regulators and effectors of microglial activation, and assays of these oxidants can be used as a measure of acute and chronic activation of microglial cells. Here we describe quick methods to assess the production of superoxide, hydrogen peroxide, nitric oxide, and peroxynitrite by microglia.

Key words

Reactive oxygen species Reactive nitrogen species Superoxide Hydrogen peroxide Nitric oxide Peroxynitrite Phagocytic NADPH oxidase Inducible nitric oxide synthase 3-Nitrotyrosine 

Abbreviations

iNOS

Inducible NOS

LPS

Lipopolysaccharide

LTA

Lipoteichoic acid

nNOS

Neuronal NOS

NO

Nitric oxide

NOS

Nitric oxide synthase

PHOX

Phagocytic NADPH oxidase

RNS

Reactive nitrogen species

RONS

Reactive oxygen and nitrogen species

ROS

Reactive oxygen species

Notes

Acknowledgement

Relevant research in our laboratory has been funded by the Wellcome Trust (Grant RG50995).

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Urte Neniskyte
    • 1
  • Guy C. Brown
    • 1
  1. 1.University of CambridgeCambridgeUK

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