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Bone Marrow Stem Cells

  • Minh Ngoc Duong
  • Yu-Ting Ma
  • Ray C. J. Chiu
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1036)

Abstract

The “mesenchymal stem cells (MSCs)” are cells adherent in the bone marrow, which can be isolated to induce differentiation. In contrast to the “embryonic stem cells” whose goal is to develop a new organism, the “MSC adult stem cells” can participate in tissue growth and repair throughout postnatal life. Addition of 5-azacytidine to MSCs in vitro induces the gradual increase in cellular size and begins spontaneous beatings, thereafter differentiating into cardiomyocytes. The “Methods” and “Protocols” to induce structural and functional maturations of MSCs, thus to achieve “Cellular Cardiomyoplasty,” are described. With appropriate media, differentiations of MSCs to various kinds of cells such as chondrocytes, osteocytes, and adipocytes are also achievable.

Key words

Cellular cardiomyoplasty Bone marrow stem cells (BMSC) Mesenchymal stem cell (MSC) MSC isolation and culture MSC differentiation 5-Azacytidine Connexin 43 Troponin I-c Transfection LacZ DAPI Echocardiography 

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Minh Ngoc Duong
    • 1
  • Yu-Ting Ma
    • 1
  • Ray C. J. Chiu
    • 1
  1. 1.Department of SurgeryMcGill University Health Center, McGill UniversityMontrealCanada

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