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Isolation and Propagation of Colon Cancer Stem Cells

  • Pramudita R. Prasetyanti
  • Cheryl Zimberlin
  • Felipe De Sousa E. Melo
  • Jan Paul Medema
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1035)

Abstract

The design of tissue culture conditions that faithfully reproduce the characteristics of cells in their native environment remains one of the main challenges of cancer stem cell (CSC) biology. Here we describe a detailed methodology for the isolation and expansion of both human colon CSCs and mouse intestinal adenoma together with a brief differentiation and coculture method that proved to be valuable to study the concept of CSCs plasticity.

Key words

Colorectal cancer Adenoma Cancer stem cells CD133 Wnt signaling Ultralow adherent EGF Isolation 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Pramudita R. Prasetyanti
    • 1
  • Cheryl Zimberlin
    • 1
  • Felipe De Sousa E. Melo
    • 1
  • Jan Paul Medema
    • 1
  1. 1.Laboratory of Experimental Oncology and Radiobiology Center for Experimental Molecular MedicineAcademic Medical CenterAmsterdamThe Netherlands

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