Isolation and Propagation of Colon Cancer Stem Cells
The design of tissue culture conditions that faithfully reproduce the characteristics of cells in their native environment remains one of the main challenges of cancer stem cell (CSC) biology. Here we describe a detailed methodology for the isolation and expansion of both human colon CSCs and mouse intestinal adenoma together with a brief differentiation and coculture method that proved to be valuable to study the concept of CSCs plasticity.
Key wordsColorectal cancer Adenoma Cancer stem cells CD133 Wnt signaling Ultralow adherent EGF Isolation
- 2.Huelsken MM, Wakamiya J, Ding J, van de Wetering M, Clevers H et al (2004) Beta-catenin regulates Cripto expression during embryogenesis and tumor formation. Eur J Cell Biol 83:31Google Scholar