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HLA Typing by Sequence-Specific Primers

  • Mike Bunce
  • Ben Passey
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1034)

Abstract

HLA typing by sequence-specific primers (PCR-SSP) is a commonly used technique in HLA typing in which multiple pairs of cis-located allele-specific primers are used to determine the alleles present in a given DNA sample. Although the technique is around two decades old, it still offers a relatively straight forward way of typing and has the benefit of using commonly available laboratory equipment. Here we describe the background of the PCR-SSP, how to design and validate reactions, and common problems that arise.

Key words

Polymerase chain reaction HLA genotyping Sequence-specific primers PCR-SSP Amplification refractory mutation system ARMS 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Mike Bunce
    • 1
  • Ben Passey
    • 1
  1. 1.Biofortuna LtdBromboroughUK

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