HLA Typing by Sequence-Specific Primers
HLA typing by sequence-specific primers (PCR-SSP) is a commonly used technique in HLA typing in which multiple pairs of cis-located allele-specific primers are used to determine the alleles present in a given DNA sample. Although the technique is around two decades old, it still offers a relatively straight forward way of typing and has the benefit of using commonly available laboratory equipment. Here we describe the background of the PCR-SSP, how to design and validate reactions, and common problems that arise.
Key wordsPolymerase chain reaction HLA genotyping Sequence-specific primers PCR-SSP Amplification refractory mutation system ARMS
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