Sedimentation Velocity Analytical Ultracentrifugation in Hydrogenated and Deuterated Solvents for the Characterization of Membrane Proteins

  • Aline Le Roy
  • Hugues Nury
  • Benjamin Wiseman
  • Jonathan Sarwan
  • Jean-Michel Jault
  • Christine Ebel
Part of the Methods in Molecular Biology book series (MIMB, volume 1033)


This chapter is a step-by-step protocol for setting up, realizing, and analyzing sedimentation velocity experiments in hydrogenated and deuterated solvents, in the context of the characterization of membrane protein, in terms of homogeneity, association state, and amount of bound detergent, based on a real case study of the membrane protein BmrA solubilized in n-Dodecyl-β-d-Maltopyranoside) detergent.

Key words

Sedimentation velocity Analytical ultracentrifugation Membrane proteins Homogeneity Association state Bound detergent BmrA Detergent Heavy water D2SEDFIT 



This work used the AUC platform of the Grenoble Instruct centre (ISBG; UMS 3518 CNRS-CEA-UJF-EMBL) with support from FRISBI (ANR-10-INSB-05-02) and GRAL (ANR-10-LABX-49-01) within the Grenoble Partnership for Structural Biology (PSB).


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Aline Le Roy
    • 1
  • Hugues Nury
    • 1
  • Benjamin Wiseman
    • 1
  • Jonathan Sarwan
    • 1
  • Jean-Michel Jault
    • 1
  • Christine Ebel
    • 1
  1. 1.Institut de Biologie Structurale, CEAGrenobleFrance

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