Quantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass Spectrometry

  • Cagakan Özbalci
  • Timo Sachsenheimer
  • Britta Brügger
Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1033)

Abstract

Lipid analysis performed by nano-electrospray ionization mass spectrometry is a highly sensitive method for quantification of lipids including all lipid species of a given lipid class. Various instrumental setups are used for quantitative lipid analysis, including different modes of ionization, separation, and detection. Here we describe a work-flow for the rapid and quantitative analysis of lipid species from cellular membranes by direct infusion of lipid extracts to a nano-electrospray ionization triple quadrupole/linear ion trap mass spectrometer.

Key words

Lipidomics Nano-electrospray ionization mass spectrometry QTRAP 5500 LipidView Quantitative lipid analysis Lipid standards 
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Notes

Acknowledgment

This work was supported by a grant of the Deutsche Forschungsgemeinschaft (SFB/TRR83). B.B. is an investigator of the CellNetworks Cluster of Excellence (EXC81).

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Cagakan Özbalci
    • 1
  • Timo Sachsenheimer
    • 1
  • Britta Brügger
    • 1
  1. 1.Heidelberg University Biochemistry Center (BZH)HeidelbergGermany

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