Selecting and Characterizing Drug-Resistant Hepatitis C Virus Replicon

  • Inge Vliegen
  • Leen Delang
  • Johan Neyts
Part of the Methods in Molecular Biology book series (MIMB, volume 1030)


The current standard of care (SOC) for hepatitis C virus (HCV) genotype 1-infected patients consists of telaprevir or boceprevir in addition to pegylated interferon and ribavirin treatment. Other selective inhibitors of HCV replication are being developed. Drug pressure may, depending on the class of inhibitors, (rapidly) select for drug-resistant variants. Here we describe four different approaches to select in vitro for drug-resistant HCV subgenomic replicons.

Key words

Drug resistance HCV Replicon Genotyping Phenotyping 


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Inge Vliegen
    • 1
  • Leen Delang
    • 1
  • Johan Neyts
    • 1
  1. 1.Rega Institute for Medical ResearchLeuvenBelgium

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