Plasmodium berghei ANKA (PbA) Infection of C57BL/6J Mice: A Model of Severe Malaria

  • Marcela Montes de Oca
  • Christian Engwerda
  • Ashraful Haque
Part of the Methods in Molecular Biology book series (MIMB, volume 1031)


The term “severe malaria” refers to a wide spectrum of syndromes in Plasmodium-infected humans including cerebral malaria (CM), respiratory distress, severe anemia, liver dysfunction, and hypoglycemia. Mouse models have been employed to further our understanding of the pathology and immune responses that occur during Plasmodium infection. Evidence of brain, liver, lung, and spleen pathology, as well as anemia and tissue-sequestration of parasites, has been reported in various strains of inbred mice. While no single mouse model mimics all the various clinical manifestations of severe malaria in humans, here we describe a detailed protocol for Plasmodium berghei ANKA infection of C57BL/6J mice. For many years, this model has been referred to as “experimental cerebral malaria,” but in fact recapitulates many of the symptoms and pathologies observed in most severe malaria syndromes.

Key words

Mouse models Malaria Plasmodium Blood-stage Disease Experimental cerebral malaria Pathology 


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Marcela Montes de Oca
    • 1
  • Christian Engwerda
    • 1
  • Ashraful Haque
    • 2
  1. 1.Immunology and Infection Laboratory, Queensland Institute of Medical ResearchHerstonAustralia
  2. 2.Malaria Immunology LaboratoryQueensland Institute of Medical ResearchHerstonAustralia

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