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Delayed-Type Hypersensitivity Models in Mice

  • Irving C. Allen
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1031)

Abstract

Delayed-type hypersensitivity (DTH) is a useful approach for evaluating cell-mediated immune responses associated with Th1 reactivity. The DTH reaction is divided into the afferent and efferent phases. During the afferent phase of this model, mice are typically immunized by subcutaneous injection with a specific hapten or antigen in its chemically reactive state and emulsified with an adjuvant. The efferent phase is typically initiated 5–12 days after sensitization, whereby the previously sensitized mice are challenged by either subcutaneous footpad injection or intradermal ear injection. The DTH response is evaluated 24 h post challenge. Here, we describe a common protocol for the induction and assessment of the DTH reaction in mice using keyhole limpet hemocyanin.

Key words

CHS Contact hypersensitivity DTH Intradermal injection In vivo Keyhole limpet hemocyanin KLH Th1 Th2 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Irving C. Allen
    • 1
  1. 1.Department of Biomedical Sciences and Pathobiology, Virginia- Maryland Regional College of Veterinary MedicineVirginia Polytechnic Institute and State UniversityBlacksburgUSA

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