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Circulating MicroRNAs in the Cerebrospinal Fluid of Patients with Brain Diseases

  • Akira Machida
  • Takuya Ohkubo
  • Takanori Yokota
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1024)

Abstract

MicroRNAs (miRNAs) are small, noncoding regulatory RNAs that regulate gene expression at the ­posttranscriptional level. Although circulating miRNAs in human body fluids have recently been recognized as disease biomarkers, especially in the field of oncology, little is known about the miRNAs in cerebrospinal fluid (CSF). This chapter describes the feasibility of miRNAs in CSF as biomarkers for the diagnosis of brain diseases and the methods of miRNA isolation from CSF.

Key words

microRNA Cerebrospinal fluid Neurodegenerative disease Biomarkers Internal control miRNA in CSF 

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Akira Machida
    • 1
  • Takuya Ohkubo
    • 1
  • Takanori Yokota
    • 1
  1. 1.Department of Neurology and Neurological Science, Graduate School of MedicineTokyo Medical and Dental UniversityTokyoJapan

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