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Encapsulation and Culture of Mammalian Cells Including Corneal Cells in Alginate Hydrogels

  • Nicola C. Hunt
  • Liam M. Grover
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1014)

Abstract

The potential of cell therapy for the regeneration of diseased and damaged tissues is now widely ­recognized. As a consequence there is a demand for the development of novel systems that can deliver cells to a particular location, maintaining viability, and then degrade at a predictable rate to release the cells into the surrounding tissues. Hydrogels have attracted much attention in this area, as the hydrogel structure provides an environment that is akin to that of the extracellular matrix. One widely investigated hydrogel is alginate, which has been used for cell encapsulation for more than 30 years. Alginate gels have the potential to be used as 3D cell culture systems and as prosthetic materials, both are applied to regeneration of the cornea. Here, we describe an alginate-based process that has been used for encapsulation of mammalian cells including corneal cells, with high levels of viability, and which allows subsequent retrieval of cell cultures for further characterization.

Key words

Alginate hydrogels Biopolymers Hydrocolloids Mammalian cells Corneal cells Cell encapsulation 

Notes

Acknowledgments

The work reported in this book chapter was funded by the EU (FP6 NanoBioTact and FP7 NanoBioTouch). The authors would like to thank Michele Marigo (University of Birmingham) for the preparation of Fig. 1.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Nicola C. Hunt
    • 1
  • Liam M. Grover
    • 1
  1. 1.School of Chemical EngineeringUniversity of BirminghamBirminghamUK

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