The Myc Gene pp 213-219

Part of the Methods in Molecular Biology book series (MIMB, volume 1012)

Studying Myc’s Role in Metabolism Regulation

  • Anne Le
  • Chi V. Dang
Protocol

Abstract

The MYC oncogene encodes a master transcription factor, Myc, which regulates genes involved in ribosome biogenesis, lipid synthesis, nucleic acid synthesis, intermediary metabolism, and cell growth and proliferation. The genomics of Myc target genes has been well-established through global mapping of Myc binding sites in a variety of different cancer cell lines. These studies highlight the importance of Myc in regulating glucose and glutamine metabolism as well as mitochondrial and ribosomal biogenesis. These genomic studies, however, only become relevant with the companion metabolic studies using a variety of methods to measure oxygen consumption, glucose uptake, or metabolic pathways based on 13C-labeled glucose or glutamine uptake. These methods are described herein.

Key words

MYC Cancer metabolism Oxygen consumption Glucose uptake Stable isotope resolved metabolomics (SIRM) 13C-labeled glucose or glutamine 

Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Anne Le
    • 1
  • Chi V. Dang
    • 2
  1. 1.Department of Pathology, Sol Goldman Pancreatic Cancer Research CenterJohns Hopkins University School of MedicineBaltimoreUSA
  2. 2.Abramson Cancer Center, Abramson Family Cancer Research Institute, Perelman School of MedicineUniversity of PennsylvaniaPhiladelphiaUSA

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