Mass Spectrometry Data Analysis in Proteomics pp 359-377

Part of the Methods in Molecular Biology book series (MIMB, volume 1007)

Proteomic Strategies to Characterize Signaling Pathways

  • H. C. Harsha
  • Sneha M. Pinto
  • Akhilesh Pandey
Protocol

Abstract

Cells respond to external stimuli by transducing signals through a series of intracellular molecules and eliciting an appropriate response. The cascade of events through which the signals are transduced include post-translational modifications such as phosphorylation and ubiquitylation in addition to formation of multi-protein complexes. Improvements in biological mass spectrometry and protein/peptide microarray technology have tremendously improved our ability to probe proteins, protein complexes, and signaling pathways in a high-throughput fashion. Today, a single mass spectrometry-based investigation of a signaling pathway has the potential to uncover the large majority of known signaling intermediates painstakingly characterized over decades in addition to discovering a number of novel ones. Here, we discuss various proteomic strategies to characterize signaling pathways and provide protocols for phosphoproteomic analysis.

Key words

Signaling pathways Mass spectrometry Phosphorylation Enrichment of phosphoproteomes Quantitative phosphoproteomics Cancer 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • H. C. Harsha
    • 1
  • Sneha M. Pinto
    • 1
  • Akhilesh Pandey
    • 2
  1. 1.Institute of BioinformaticsInternational Technology ParkBangaloreIndia
  2. 2.Departments of Biological Chemistry, Pathology, and Oncology, McKusick-Nathans Institute of Genetic MedicineJohns Hopkins UniversityBaltimoreUSA

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