Necrosis pp 67-73

Part of the Methods in Molecular Biology book series (MIMB, volume 1004)

Analysis of Pyroptosis in Bacterial Infection

  • Lia Danelishvili
  • Luiz E. Bermudez

Abstract

Eukaryotic cells undergo death by several different mechanisms: apoptosis, a cell death that prevents inflammatory response; necrosis, when the cell membrane lyses and all the intracellular content is spilled outside; and pyroptosis, a cell death that is accompanied by the release of inflammatory cytokines by the dying cells. Pyroptosis is designed to attract a nonspecific innate response to the site of infection or tumor. In this chapter, we describe the methods used to study pyroptosis in a mammalian cell. The model organism used is Mycobacterium tuberculosis, which suppresses pyroptosis by macrophages, and possibly in dendritic cells.

Key words

Pyroptosis Apoptosis Necrosis Macrophages Methods Caspase-1 Inflammatory response 

Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Lia Danelishvili
    • 1
  • Luiz E. Bermudez
    • 1
    • 2
    • 3
  1. 1.Department of Biomedical Sciences, College of Veterinary MedicineOregon State UniversityCorvallisUSA
  2. 2.Department of Microbiology, College of ScienceOregon State UniversityCorvallisUSA
  3. 3.Program of Molecular and Cell BiologyOregon State UniversityCorvallisUSA

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