Necrosis pp 203-213 | Cite as

Modeling Calcium-Overload Mediated Necrosis in Drosophila

  • Kai Liu
  • Yuhong Li
  • Lei Liu
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1004)

Abstract

Necrotic cell death (necrosis) occurs in many acute-onset diseases. However, our poor understanding of its mechanism has greatly limited medical interventions. Here we describe two methods to establish necrosis models in Drosophila. Our strategy is to overload calcium by expression of leaky cation channels.

Key words

Necrotic cell death Necrosis Leaky cation channel Cell death model Drosophila 
This is a preview of subscription content, log in to check access.

References

  1. 1.
    Yuan J (2009) Neuroprotective strategies targeting apoptotic and necrotic cell death for stroke. Apoptosis 14:469–477CrossRefPubMedCentralPubMedGoogle Scholar
  2. 2.
    Zong WX, Thompson CB (2006) Necrotic death as a cell fate. Genes Dev 20:1–15CrossRefPubMedGoogle Scholar
  3. 3.
    Chalfie M, Wolinsky E (1990) The identification and suppression of inherited neurodegeneration in Caenorhabditis elegans. Nature 345:410–416CrossRefPubMedGoogle Scholar
  4. 4.
    Xu K, Tavernarakis N, Driscoll M (2001) Necrotic cell death in C. elegans requires the function of calreticulin and regulators of Ca(2+) release from the endoplasmic reticulum. Neuron 31:957–971CrossRefPubMedGoogle Scholar
  5. 5.
    Syntichaki P, Xu K, Driscoll M, Tavernarakis N (2002) Specific aspartyl and calpain proteases are required for neurodegeneration in C. elegans. Nature 419:939–944CrossRefPubMedGoogle Scholar
  6. 6.
    Adams CM, Snyder PM, Price MP, Welsh MJ (1998) Protons activate brain Na+ channel 1 by inducing a conformational change that exposes a residue associated with neurodegeneration. J Biol Chem 273:30204–30207CrossRefPubMedGoogle Scholar
  7. 7.
    Kohda K, Wang Y, Yuzaki M (2000) Mutation of a glutamate receptor motif reveals its role in gating and delta2 receptor channel properties. Nat Neurosci 3:315–322CrossRefPubMedGoogle Scholar
  8. 8.
    Rubin GM, Spradling AC (1982) Genetic transformation of Drosophila with transposable element vectors. Science 218:348–353CrossRefPubMedGoogle Scholar
  9. 9.
    Spradling AC, Rubin GM (1982) Transposition of cloned P elements into Drosophila germ line chromosomes. Science 218:341–347CrossRefPubMedGoogle Scholar
  10. 10.
    Cardona A et al (2010) An integrated micro- and macroarchitectural analysis of the Drosophila brain by computer-assisted serial section electron microscopy. PLoS Biol 8(10):e1000502CrossRefPubMedCentralPubMedGoogle Scholar
  11. 11.
    Fan Y, Bergmann A (2009) The cleaved-Caspase-3 antibody is a marker of Caspase-9-like DRONC activity in Drosophila. Cell Death Differ 17:534–539CrossRefPubMedCentralPubMedGoogle Scholar
  12. 12.
    Wolff T (2010) Cryosectioning and immunocytochemistry of adult Drosophila eye sections. Cold Spring Harb Protoc 2010:pdb prot5370Google Scholar
  13. 13.
    Hutchins JB, Barger SW (1998) Why neurons die: cell death in the nervous system. Anat Rec 253:79–90CrossRefPubMedGoogle Scholar
  14. 14.
    Giordano E, Rendina R, Peluso I, Furia M (2002) RNAi triggered by symmetrically transcribed transgenes in Drosophila melanogaster. Genetics 160:637–648PubMedCentralPubMedGoogle Scholar
  15. 15.
    Hay BA, Wolff T, Rubin GM (1994) Expression of baculovirus P35 prevents cell death in Drosophila. Development 120:2121–2129PubMedGoogle Scholar
  16. 16.
    Steller H (2008) Regulation of apoptosis in Drosophila. Cell Death Differ 15:1132–1138CrossRefPubMedGoogle Scholar
  17. 17.
    White K et al (1994) Genetic control of programmed cell death in Drosophila. Science 264:677–683CrossRefPubMedGoogle Scholar
  18. 18.
    Chang YY, Neufeld TP (2009) An Atg1/Atg13 complex with multiple roles in TOR-mediated autophagy regulation. Mol Biol Cell 20:2004–2014CrossRefPubMedCentralPubMedGoogle Scholar
  19. 19.
    Mondal K et al (2007) Design and isolation of temperature-sensitive mutants of Gal4 in yeast and Drosophila. J Mol Biol 370:939–950CrossRefPubMedGoogle Scholar
  20. 20.
    Eberl DF, Boekhoff-Falk G (2007) Development of Johnston’s organ in Drosophila. Int J Dev Biol 51:679–687CrossRefPubMedCentralPubMedGoogle Scholar
  21. 21.
    Loo DT, Rillema JR (1998) Measurement of cell death. Methods Cell Biol 57:251–264CrossRefPubMedGoogle Scholar
  22. 22.
    Walsh GS, Orike N, Kaplan DR, Miller FD (2004) The invulnerability of adult neurons: a critical role for p73. J Neurosci 24:9638–9647CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Kai Liu
    • 1
  • Yuhong Li
    • 1
  • Lei Liu
    • 1
  1. 1.State Key Lab of Biomembrane and Membrane Biotechnology, School of Life SciencesPeking UniversityBeijingChina

Personalised recommendations