Human Liver Bioengineering Using a Whole Liver Decellularized Bioscaffold

  • Pedro M. Baptista
  • Dipen Vyas
  • Emma Moran
  • Zhan Wang
  • Shay Soker
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1001)

Abstract

As a result of significant progress made in the last years in developing methods of whole organ decellularization techniques, organ bioengineering may now look more feasible than ever before. In this chapter, we describe in detail the necessary steps in human liver bioengineering. These include ferret liver decellularization by detergent perfusion, human liver progenitor and endothelial cell isolation, and finally, liver bioscaffold recellularization in a perfusion bioreactor.

Key words

Human liver progenitors Decellularization Scaffold Bioengineering Endothelial cells Perfusion bioreactor Whole organ Recellularization 

References

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    Baptista PM, Orlando G, Mirmalek-Sani SH et al (2009) Whole organ decellularization—a tool for bioscaffold fabrication and organ bioengineering. Conf Proc IEEE Eng Med Biol Soc 2009:6526–6529PubMedGoogle Scholar
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    Ott HC, Matthiesen TS, Goh SK et al (2008) Perfusion-decellularized matrix: using nature’s platform to engineer a bioartificial heart. Nat Med 14(2):213–221PubMedCrossRefGoogle Scholar
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    Baptista PM, Siddiqui MM, Lozier G et al (2011) The use of whole organ decellularization for the generation of a vascularized liver organoid. Hepatology 53(2):604–617PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Pedro M. Baptista
    • 1
  • Dipen Vyas
    • 1
  • Emma Moran
    • 1
  • Zhan Wang
    • 1
  • Shay Soker
    • 1
  1. 1.Wake Forest Institute for Regenerative MedicineWinston-SalemUSA

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