As a result of significant progress made in the last years in developing methods of whole organ decellularization techniques, organ bioengineering may now look more feasible than ever before. In this chapter, we describe in detail the necessary steps in human liver bioengineering. These include ferret liver decellularization by detergent perfusion, human liver progenitor and endothelial cell isolation, and finally, liver bioscaffold recellularization in a perfusion bioreactor.
Human liver progenitors Decellularization Scaffold Bioengineering Endothelial cells Perfusion bioreactor Whole organ Recellularization
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Gilbert TW, Sellaro TL, Badylak SF (2006) Decellularization of tissues and organs. Biomaterials 27(19):3675–3683PubMedGoogle Scholar
Baptista PM, Orlando G, Mirmalek-Sani SH et al (2009) Whole organ decellularization—a tool for bioscaffold fabrication and organ bioengineering. Conf Proc IEEE Eng Med Biol Soc 2009:6526–6529PubMedGoogle Scholar
Ott HC, Matthiesen TS, Goh SK et al (2008) Perfusion-decellularized matrix: using nature’s platform to engineer a bioartificial heart. Nat Med 14(2):213–221PubMedCrossRefGoogle Scholar
Baptista PM, Siddiqui MM, Lozier G et al (2011) The use of whole organ decellularization for the generation of a vascularized liver organoid. Hepatology 53(2):604–617PubMedCrossRefGoogle Scholar