Organ Regeneration pp 267-278

Part of the Methods in Molecular Biology book series (MIMB, volume 1001)

Bioengineered Skin Substitutes

  • Pedro Lei
  • Hui You
  • Stelios T. Andreadis


Bioengineered skin has great potential for use in regenerative medicine for treatment of severe wounds such as burns or chronic ulcers. Genetically modified skin substitutes have also been used as cell-based devices or “live bioreactors” to deliver therapeutics locally or systemically. Finally, these tissue constructs are used as realistic models of human skin for toxicological testing, to speed drug development and replace traditional animal-based tests in a variety of industries. Here we describe a method of generating bioengineered skin based on a natural scaffold, namely, decellularized human dermis and epidermal stem cells.

Key words

Epidermal stem cells Three-dimensional tissue constructs Keratinocytes Differentiation 


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Pedro Lei
    • 1
  • Hui You
    • 1
  • Stelios T. Andreadis
    • 1
  1. 1.Department of Chemical and Biological EngineeringUniversity at Buffalo, State University of New YorkAmherstUSA

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