Generation of Induced Pluripotent Stem Cells with CytoTune, a Non-Integrating Sendai Virus

  • Pauline T. Lieu
  • Andrew Fontes
  • Mohan C. Vemuri
  • Chad C. MacArthur
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 997)

Abstract

One of the major obstacles in generating induced pluripotent stem cells for research or downstream applications is the potential modifications of cellular genome as a result of using integrating viruses during reprogramming. Another major disadvantage of reprogramming cells with integrating vectors is that silencing and activation of transgenes are unpredictable, which may affect terminal differentiation potential and increase the risk of using iPSC-derived cells. Here we describe a protocol for the generation of induced pluripotent stem cells using a non-integrating RNA virus, Sendai virus, to efficiently generate transgene-free iPSCs starting with different cell types as well as in feeder-free conditions.

Key words

Induced pluripotent stem cells Non-integrating Sendai virus High efficiency Human cells Reprogramming Feeder free 

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Pauline T. Lieu
    • 1
  • Andrew Fontes
    • 1
  • Mohan C. Vemuri
    • 2
  • Chad C. MacArthur
    • 1
  1. 1.Primary and Stem Cell SystemsLife TechnologiesCarlsbadUSA
  2. 2.Primary and Stem Cell SystemsLife TechnologiesFrederickUSA

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