Cellular and Subcellular Nanotechnology pp 65-80

Part of the Methods in Molecular Biology book series (MIMB, volume 991)

A Method to Encapsulate Molecular Cargo Within DNA Icosahedra

  • Dhiraj Bhatia
  • Saikat Chakraborty
  • Shabana Mehtab
  • Yamuna Krishnan
Protocol

Abstract

DNA self-assembly has yielded various polyhedra based on platonic solids. DNA polyhedra can act as nanocapsules by entrapping various molecular entities from solution and could possibly find use in targeted delivery within living systems. A key requirement for encapsulation is that the polyhedron should have maximal encapsulation volume while maintaining minimum pore size. It is well known that platonic solids possess maximal encapsulation volumes. We therefore constructed an icosahedron from DNA using a modular self-assembly strategy. We describe a method to determine the functionality of DNA polyhedra as nanocapsules by encapsulating different cargo such as gold nanoparticles and functional biomolecules like FITC dextran from solution within DNA icosahedra.

Key words

DNA icosahedron Polyhedra Nanocapsules Encapsulation Gold nanoparticles FITC dextran 

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Dhiraj Bhatia
    • 1
  • Saikat Chakraborty
    • 1
  • Shabana Mehtab
    • 1
  • Yamuna Krishnan
    • 1
  1. 1.National Centre for Biological Sciences, Tata Institute of Fundamental ResearchBangaloreIndia

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