Immunoisolation of Nanoparticles Containing Endocytic Vesicles for Drug Quantitation

  • Ari Nowacek
  • Irena Kadiu
  • JoEllyn McMillan
  • Howard E. Gendelman
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 991)

Abstract

Cell-mediated nanoparticle delivery has recently emerged as an efficacious method of delivering therapeutic agents across physiological barriers. Use of cells as nanodelivery vehicles requires accurate assessment of their loading capacity and identification of intracellular compartments where nanoparticles are sequestered. This is of great interest since specific endocytic trafficking routes can ultimately influence the mode of nanoparticle release and their efficacy and function. Here, we describe a technique that allows for the isolation of individual populations of nanoparticle-containing endosomes for subsequent quantitative analysis and more accurate description of where nanoparticles are stored on a subcellular level.

Key words

Nanoparticles Immunoisolation Endosomes Subcellular trafficking Macrophage 

References

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    Kadiu I, Nowacek A, McMillan J, Gendelman HE (2011) Macrophage endocytic trafficking of antiretroviral nanoparticles. Nanomedicine (Lond) 6:975–994CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Ari Nowacek
    • 1
  • Irena Kadiu
    • 1
  • JoEllyn McMillan
    • 1
  • Howard E. Gendelman
    • 1
  1. 1.Department of Pharmacology and Experimental NeuroscienceUniversity of Nebraska Medical CenterOmahaUSA

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