Using Sequential Fluorescence and Genomic In Situ Hybridization (FISH and GISH) to Distinguish the A and C Genomes in Brassica napus
We have developed a sequential procedure with fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) that enables us to distinguish between the A and C genomes in Brassica napus and to identify certain individual chromosomes or chromosome groups within a genome. Our modified GISH technique uses a repetitive sequence in addition to the whole genome in the blocking DNA, and it is effective on meiotic and mitotic cells present in the anther material that we use.
KeywordsBrassica A genome C genome Genomic in situ hybridization GISH Fluorescence in situ hybridization FISH Meiosis BAC
E.C. Howell was supported by BBSRC. We thank S. Price for technical assistance and I.A. Parkin (AAFC, Saskatoon, Canada) for B. napus seed.