Isolation and Establishment of Hair Follicle Dermal Papilla Cell Cultures

  • Karl Gledhill
  • Aaron Gardner
  • Colin A. B. Jahoda
Part of the Methods in Molecular Biology book series (MIMB, volume 989)


The isolation of hair follicle dermal papilla cells has become an important technique in the field of cutaneous stem cell biology. These cells can be used for a number of biological and translational purposes. They are studied to identify the cellular characteristics and molecular factors that underpin the initiation, maintenance, and modulation of hair growth; to develop new human hair replacement techniques; and as a source of cells capable of being directed down a variety of different lineages. Here, we describe the isolation of hair follicle dermal papilla cells from both human and murine sources via the microdissection techniques used in our lab.

Key words

Hair follicle Dermal papilla Stem cells Microdissection End-bulb Cell culture 



The authors would like to acknowledge the photographical assistance provided by Rebecca P. Hill and Simon C. Cork in producing Figs. 1 (e–g) and 3 (respectively) and proof reading by Laura R. J. Ions. We are very grateful to DEBRA and the MRC for grant support.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Karl Gledhill
    • 1
  • Aaron Gardner
    • 1
  • Colin A. B. Jahoda
    • 1
  1. 1.School of Biological and Biomedical SciencesDurham UniversityDurhamUK

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