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Isolation and Establishment of Hair Follicle Dermal Papilla Cell Cultures

  • Karl Gledhill
  • Aaron Gardner
  • Colin A. B. Jahoda
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 989)

Abstract

The isolation of hair follicle dermal papilla cells has become an important technique in the field of cutaneous stem cell biology. These cells can be used for a number of biological and translational purposes. They are studied to identify the cellular characteristics and molecular factors that underpin the initiation, maintenance, and modulation of hair growth; to develop new human hair replacement techniques; and as a source of cells capable of being directed down a variety of different lineages. Here, we describe the isolation of hair follicle dermal papilla cells from both human and murine sources via the microdissection techniques used in our lab.

Key words

Hair follicle Dermal papilla Stem cells Microdissection End-bulb Cell culture 

Notes

Acknowledgments

The authors would like to acknowledge the photographical assistance provided by Rebecca P. Hill and Simon C. Cork in producing Figs. 1 (e–g) and 3 (respectively) and proof reading by Laura R. J. Ions. We are very grateful to DEBRA and the MRC for grant support.

References

  1. 1.
    Oliver RF, Jahoda CA (1988) Dermal-epidermal interactions. Clin Dermatol 6(4):74–82PubMedCrossRefGoogle Scholar
  2. 2.
    Driskell RR, Clavel C, Rendl M, Watt FM (2011) Hair follicle dermal papilla cells at a glance. J Cell Sci 124(8):1179–1182PubMedCrossRefGoogle Scholar
  3. 3.
    Oliver RF (1967) The experimental induction of whisker growth in the hooded rat by implantation of dermal papillae. J Embryol Exp Morphol 18(1):43–51PubMedGoogle Scholar
  4. 4.
    Oliver RF (1970) The induction of hair follicle formation in the adult hooded rat by vibrissa dermal papillae. J Embryol Exp Morphol 23(1):219–236PubMedGoogle Scholar
  5. 5.
    Richardson GD, Arnott EC, Whitehouse CJ, Lawrence CM, Hole N, Jahoda CAB (2005) Cultured cells from the adult human hair follicle dermis can be directed toward adipogenic and osteogenic differentiation. J Invest Dermatol 124(5):1090–1091PubMedCrossRefGoogle Scholar
  6. 6.
    Jahoda CAB, Whitehouse J, Reynolds AJ, Hole N (2003) Hair follicle dermal cells differentiate into adipogenic and osteogenic lineages. Exp Dermatol 12(6):849–859PubMedCrossRefGoogle Scholar
  7. 7.
    Rufaut NW, Goldthorpe NT, Wildermoth JE, Wallace OAM (2006) Myogenic differentiation of dermal papilla cells from bovine skin. J Cell Physiol 209(3):959–966PubMedCrossRefGoogle Scholar
  8. 8.
    Hunt DP, Morris PN, Sterling J, Anderson JA, Joannides A, Jahoda C, Compston A, Chandran S (2008) A highly enriched niche of precursor cells with neuronal and glial potential within the hair follicle dermal papilla of adult skin. Stem Cells 26(1):163–172PubMedCrossRefGoogle Scholar
  9. 9.
    Higgins CA, Itoh M, Inoue K, Richardson GD, Jahoda CAB, Christiano AM (2012) Reprogramming of human hair follicle dermal papilla cells into induced pluripotent stem cells. J Invest Dermatol 132(6):1725–1727PubMedCrossRefGoogle Scholar
  10. 10.
    Lako M, Armstrong L, Cairns PM, Harris S, Hole N, Jahoda CA (2002) Hair follicle dermal cells repopulate the mouse haematopoietic system. J Cell Sci 115(20):3967–3974PubMedCrossRefGoogle Scholar
  11. 11.
    Jahoda CA, Reynolds AJ, Chaponnier C, Forester JC, Gabbiani G (1991) Smooth muscle alpha-actin is a marker for hair follicle dermis in vivo and in vitro. J Cell Sci 99(3):627–636PubMedGoogle Scholar
  12. 12.
    Kishimoto J, Ehama R, Wu L, Jiang S, Jiang N, Burgeson RE (1999) Selective activation of the versican promoter by epithelial- mesenchymal interactions during hair follicle development. Proc Natl Acad Sci U S A 96(13):7336–7341PubMedCrossRefGoogle Scholar
  13. 13.
    McElwee KJ, Kissling S, Wenzel E, Huth A, Hoffmann R (2003) Cultured peribulbar dermal sheath cells can induce hair follicle development and contribute to the dermal sheath and dermal papilla. J Invest Dermatol 121(6):1267–1275PubMedCrossRefGoogle Scholar
  14. 14.
    Rendl M, Lewis L, Fuchs E (2005) Molecular dissection of mesenchymal-epithelial interactions in the hair follicle. PLoS Biol 3(11):e331PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Karl Gledhill
    • 1
  • Aaron Gardner
    • 1
  • Colin A. B. Jahoda
    • 1
  1. 1.School of Biological and Biomedical SciencesDurham UniversityDurhamUK

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