Glycosylation Engineering of Biopharmaceuticals pp 199-209

Part of the Methods in Molecular Biology book series (MIMB, volume 988)

Characterization of Glycoprotein Biopharmaceutical Products by Caliper LC90 CE-SDS Gel Technology

  • Grace Chen
  • Sha Ha
  • Richard R. Rustandi
Protocol

Abstract

Over the last decade, science has greatly improved in the area of protein sizing and characterization. Efficient high-throughput methods are now available to substitute for the traditional labor-intensive SDS-PAGE methods, which alternatively take days to analyze a very limited number of samples. Currently, PerkinElmer® (Caliper) has designed an automated chip-based fluorescence detection method capable of analyzing proteins in minutes with sensitivity similar to standard SDS-PAGE. Here, we describe the use and implementation of this technology to characterize and screen a large number of formulations of target glycoproteins in the 14–200 kDa molecular weight range.

Key words

Caliper LC90 Caliper GX/GXII CE-SDS gel N-linked glycan occupancy High-throughput methods Molecular weight Glycoprotein characterization Monoclonal antibody EPO Fc-fusion protein 

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Grace Chen
    • 1
  • Sha Ha
    • 1
  • Richard R. Rustandi
    • 1
  1. 1.Vaccine Analytical DevelopmentMerck Research LaboratoriesWest PointUSA

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