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Comprehensive Hematopoietic Stem Cell Isolation Methods

  • Kyle RectorEmail author
  • Yi Liu
  • Gary Van Zant
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 976)

Abstract

The use of flow cytometry has been critical in establishing methods to isolate and characterize hematopoietic stem cells (HSCs) and their progenitors. For more than 30 years, researchers have been uncovering novel markers that when used in combination significantly enhance the purification of HSCs from murine and human bone marrow. The complex interface between HSCs, the lymphohematopoietic system, and their niches, has made identification of HSC markers critical to understanding their biological nature, more so than other adult stem cell populations. Here we review the phenotypic markers and strategies used to purify HSCs, the appropriateness of using these markers for comparisons of HSC function at different stages of ontogeny, and their utility in defining the lineage bias in the HSC compartment.

Keywords

Hematopoietic stem cells Isolation methods Phenotypic markers Lineage bias 

Notes

Acknowledgments

We would like to thank Paula Thomason for her expert editorial assistance in the preparation of the manuscript and Ying Liang for her critical review.

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Departments of Physiology, Markey Cancer CenterUniversity of KentuckyLexingtonUSA
  2. 2.Department of Physiology, Markey Cancer CenterUniversity of KentuckyLexingtonUSA

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