Abstract
The evaluation of respiratory parameters often requires the use of anesthetics (that depress the neural network controlling respiration), and/or ways to restrain the animal’s mobility (that produces a stress-dependent increase of respiration). Consequently, the establishment of plethysmography represented an invaluable technique in respiratory physiology. Plethysmography, indeed, allows the assessment of ventilatory parameters on living, unanesthetized, and unrestrained animals. The conception of the barometric plethysmography relies on the fact that an animal placed inside a hermetically closed chamber generates through its breathing a fluctuation of pressure in the chamber than can be recorded. Thus, the respiratory frequency and the tidal volume can be directly measured, while the animal’s ventilation is calculated indirectly by the multiplication of these two parameters. In our hands, plethysmography was a key tool to investigate the impact of erythropoietin (Epo) on the neural control of hypoxic ventilation in mice.
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References
Soliz J, Gassmann M, Joseph V (2007) J Physiol 583:329–336
Soliz J, Joseph V, Soulage C, Becskei C, Vogel J, Pequignot JM, Ogunshola O, Gassmann M (2005) J Physiol 568:559–571
Joseph V, Soliz J, Pequignot J, Sempore B, Cottet-Emard JM, Dalmaz Y, Favier R, Spielvogel H, Pequignot JM (2000) Am J Physiol Regul Integr Comp Physiol 278:R806–R816
Tashiro N, Kataoka M, Ozawa K, Ikeda T (2007) J Am Assoc Lab Anim Sci 46:81–85
Vargas MH, Sommer B, Bazan-Perkins B, Montano LM (2010) Vet Res Commun 34:589–596
Kirschvink N, Leemans J, Delvaux F, Snaps F, Clercx C, Gustin P (2007) Vet J 173:343–352
Murphy DJ, Renninger JP, Schramek D (2010) J Pharmacol Toxicol Methods 62:47–53
Halloy DJ, Kirschvink NA, Vincke GL, Hamoir JN, Delvaux FH, Gustin PG (2004) Vet J 168:276–284
Bedenice D, Bar-Yishay E, Ingenito EP, Tsai L, Mazan MR, Hoffman AM (2004) Am J Vet Res 65:1259–1264
Nolen-Walston RD, Kuehn H, Boston RC, Mazan MR, Wilkins PA, Bruns S, Hoffman AM (2009) J Vet Intern Med 23:631–635
Iizuka H, Sasaki K, Odagiri N, Obo M, Imaizumi M, Atai H (2010) J Toxicol Sci 35:863–870
Soliz J, Soulage C, Hermann DM, Gassmann M (2007) Am J Physiol Regul Integr Comp Physiol 293:R1702–R1710
Joseph V, Soliz J, Soria R, Pequignot J, Favier R, Spielvogel H, Pequignot JM (2002) Am J Physiol Regul Integr Comp Physiol 282:R765–R773
Drorbaugh JE, Fenn WO (1955) Pediatrics 16:81–87
Menuet C, Kourdougli N, Hilaire G, Voituron N (2011) J Appl Physiol 110:1572–1581
Wiessner C, Allegrini PR, Ekatodramis D, Jewell UR, Stallmach T, Gassmann M (2001) J Cereb Blood Flow Metab 21:857–864
Ruschitzka FT, Wenger RH, Stallmach T, Quaschning T, de Wit C, Wagner K, Labugger R, Kelm M, Noll G, Rulicke T, Shaw S, Lindberg RL, Rodenwaldt B, Lutz H, Bauer C, Luscher TF, Gassmann M (2000) Proc Natl Acad Sci U S A 97:11609–11613
Acknowledgments
The authors would like to thank Drs Vincent Joseph and Cécile Julien as well as Mr Raphaël Lavoie and Miss Hanan Khemiri for helpful discussions and valuable advices. J.S. is supported by the Respiratory Health Network of the FRSQ (Fonds de la Recherche en Santé du Québec), the Foundation of Stars for the Children’s health research, the Molly Towell Perinatal Research Foundation (MTPRF). M.G. is supported by the Zurich Center for Integrative Human Physiology (ZIHP).
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Seaborn, T., Gassmann, M., Soliz, J. (2013). Using Plethysmography to Determine Erythropoietin’s Impact on Neural Control of Ventilation. In: Ghezzi, P., Cerami, A. (eds) Tissue-Protective Cytokines. Methods in Molecular Biology, vol 982. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-308-4_18
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DOI: https://doi.org/10.1007/978-1-62703-308-4_18
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