Identification and Verification of microRNAs by High-Throughput Sequencing
High-throughput sequencing methods have become invaluable for detection and analysis of small RNAs. The results are millions of sequences that need to be carefully analyzed by computational methods and preferentially verified by different experimental techniques. Here we describe how to use high-throughput sequencing followed by bioinformatics and northern blot to identify one particular class of small RNA, microRNAs.
Key wordsHigh-throughput sequencing MicroRNA Northern blot Bioinformatics Small RNA
This work was supported by The Swedish Research Council (to F.S. and to Uppsala RNA Research Center) and The Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (FORMAS) to F.S. The authors would like to acknowledge support from Science for Life Laboratory, the national infrastructure SNISS, and UPPMAX for providing assistance in massively parallel sequencing and computational infrastructure.
- 7.Martin M (2011) Cutadapt removes adapter sequences from high-throughput sequencing reads. EMBnet J 17:10–12Google Scholar