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Identification and Verification of microRNAs by High-Throughput Sequencing

  • Jimmie Hällman
  • Lotta Avesson
  • Johan Reimegård
  • Max Käller
  • Fredrik SöderbomEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 983)

Abstract

High-throughput sequencing methods have become invaluable for detection and analysis of small RNAs. The results are millions of sequences that need to be carefully analyzed by computational methods and preferentially verified by different experimental techniques. Here we describe how to use high-throughput sequencing followed by bioinformatics and northern blot to identify one particular class of small RNA, microRNAs.

Key words

High-throughput sequencing MicroRNA Northern blot Bioinformatics Small RNA 

Notes

Acknowledgements

This work was supported by The Swedish Research Council (to F.S. and to Uppsala RNA Research Center) and The Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (FORMAS) to F.S. The authors would like to acknowledge support from Science for Life Laboratory, the national infrastructure SNISS, and UPPMAX for providing assistance in massively parallel sequencing and computational infrastructure.

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Jimmie Hällman
    • 1
  • Lotta Avesson
    • 2
    • 3
  • Johan Reimegård
    • 1
  • Max Käller
    • 1
  • Fredrik Söderbom
    • 4
    Email author
  1. 1.School of Biotechnology, Division of Gene Technology, Science for Life Laboratory (SciLifeLab Stockholm)KTH Royal Institute of TechnologySolnaSweden
  2. 2.Department of Molecular Biology, Biomedical CenterSwedish University of Agricultural SciencesUppsalaSweden
  3. 3.Garvan Institute of Medical ResearchDarlinghurstAustralia
  4. 4.Department of Cell and Molecular Biology, Swedish University of Agricultural SciencesUppsala UniversityUppsalaSweden

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