Detection of Necrosis by Release of Lactate Dehydrogenase Activity
Apoptosis and necrosis are two major forms of cell death observed in normal and disease pathologies. Although there are many assays for detection of apoptosis, relatively few assays are available for measuring necrosis. A key signature for necrotic cells is the permeabilization of the plasma membrane. This event can be quantified in tissue culture settings by measuring the release of the intracellular enzyme lactate dehydrogenase (LDH). When combined with other methods, measuring LDH release is a useful method for the detection of necrosis. In this chapter, we describe the step-by-step procedure for detection of LDH release from necrotic cells using a microtiter plate-based colorimetric absorbance assay.
Key wordsProgrammed necrosis Necroptosis Membrane leakage Lactate dehydrogenase
This work was supported by NIH grants (AI083497 and AI088502) to F. K-M. C. K. M. is supported by a postdoctoral fellowship from the Uehara Memorial Foundation, Japan. MJDR is supported by a CONICET fellowship from Argentina.