Flow Cytometric Measurement of SLAM-Associated Protein and X-Linked Inhibitor of Apoptosis

  • Rebecca A. Marsh
  • Jack J. Bleesing
  • Alexandra H. Filipovich
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 979)

Abstract

Flow cytometry is a valuable tool for the detection and characterization of proteins expressed by individual cells. Flow cytometry can be used to measure cell expression of 2 intracellular proteins that are involved in the regulation of immune homeostasis, SLAM-associated protein (SAP) and X-linked inhibitor of apoptosis (XIAP). These proteins are defective in patients with the immune deficiency X-linked lymphoproliferative disease (XLP), due to mutations in the SH2D1A and XIAP/BIRC4 genes, respectively (Coffey et al. Nat Genet 20:129-135 1998; Nichols et al. Proc Natl Acad Sci U S A 95:13765-13770, 1998; Sayos et al. Nature 395:462-469, 1998; Rigaud et al. Nature 444:110-114, 2006). This procedure describes a technique that can be efficiently used to detect SAP and XIAP by flow cytometry.

Key words

X-linked Lymphoproliferative Disease (XLP) SLAM-Associated Protein (SAP) X-linked Inhibitor of Apoptosis (XIAP) 

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Rebecca A. Marsh
    • 1
  • Jack J. Bleesing
    • 1
  • Alexandra H. Filipovich
    • 1
  1. 1.Division of Bone Marrow Transplantation and Immune Deficiency, Cancer and Blood Diseases InstituteCincinnati Children’s Hospital Medical CenterCincinnatiUSA

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