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A Modified Yeast One-Hybrid System for Genome-Wide Identification of Transcription Factor Binding Sites

Part of the Methods in Molecular Biology book series (MIMB,volume 977)

Abstract

The yeast one-hybrid system is a powerful genetic method to identify DNA–protein interactions, but there is a major limitation inherent to the system. Namely, frequency of false positives generated by yeast endogenous transcription factors has been thought to be higher than that of true positives by orders of magnitude. However, our modification efficiently can eliminate the false positives. When compared to the other methods for the analysis of DNA–protein interactions on a genome-wide scale, a modified yeast one-hybrid system offers several advantages including low initial and running cost, large-scale output, and easy handling.

Key words

  • Yeast one-hybrid assay
  • Transcription factor
  • DNA–protein interaction
  • Binding site
  • DNA element
  • Target gene
  • Genome wide
  • Chromatin
  • Chromatin immunoprecipitation
  • ChIP-on-chip

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Acknowledgment

This work was supported by the Research Grant for the faculty of science special grant for promoting scientific research at Toho University.

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Yanai, K. (2013). A Modified Yeast One-Hybrid System for Genome-Wide Identification of Transcription Factor Binding Sites. In: Bina, M. (eds) Gene Regulation. Methods in Molecular Biology, vol 977. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-284-1_10

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  • DOI: https://doi.org/10.1007/978-1-62703-284-1_10

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-283-4

  • Online ISBN: 978-1-62703-284-1

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