Array Comparative Genomic Hybridization

Volume 973 of the series Methods in Molecular Biology pp 39-54


Epigenomics: Sequencing the Methylome

  • Martin HirstAffiliated withDepartment of Microbiology and Immunology, Centre for High-Throughput Biology, University of British ColumbiaBC Cancer Agency, Canada’s Michael Smith Genome Sciences Centre Email author 

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DNA methylation patterns are increasingly surveyed through methods that utilize massively parallel sequencing. Sequence-based assays developed to detect DNA methylation can be broadly divided into those that depend on affinity enrichment, chemical conversion, or enzymatic restriction. The DNA fragments resulting from these methods are uniformly subjected to library construction and massively parallel sequencing. The sequence reads are subsequently aligned to a reference genome and subjected to specialized analytical tools to extract the underlying methylation signature. This chapter will outline these emerging techniques.

Key words

Epigenetics Epigenomics DNA methylation Massively parallel sequencing