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Antitumor Vaccination with Synthetic mRNA: Strategies for In Vitro and In Vivo Preclinical Studies

  • Mustafa Diken
  • Sebastian Kreiter
  • Abderraouf Selmi
  • Özlem Türeci
  • Ugur Sahin
Part of the Methods in Molecular Biology book series (MIMB, volume 969)

Abstract

Synthetic antigen-encoding mRNA is increasingly exploited as a tool for delivery of genetic information of complete antigens into professional antigen presenting dendritic cells for HLA haplotype-independent antigen-specific vaccination against cancer. Two strategies for mRNA-based antitumor vaccination have emerged into the clinical setting. One is transfection of autologous dendritic cells with synthetic mRNA for adoptive transfer into the patient. The other is direct injection of naked synthetic mRNA. Both methods have proven to be feasible and safe and to elicit antigen-specific immune responses. The design of novel synthetic vaccines employing synthetic mRNA requires further in-depth investigation of its bioavailability and immune pharmacology. This chapter summarizes the state-of-art in this field and describes methods elementary for preclinical studies of mRNA-based antitumor vaccine protocols.

Key words

mRNA vaccination Dendritic cell Cancer immunotherapy Lymph node 

Notes

Acknowledgments

This research was supported by the GO-Bio funding of the German Federal Ministry of Education and Research to Ugur Sahin.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Mustafa Diken
    • 1
  • Sebastian Kreiter
    • 1
  • Abderraouf Selmi
    • 1
    • 2
  • Özlem Türeci
    • 3
  • Ugur Sahin
    • 4
    • 5
    • 6
  1. 1.Translational Oncology and Immunology (TRON) at the Johannes Gutenberg University MainzMainzGermany
  2. 2.Division of Translational and Experimental Oncology, Department of Internal Medicine IIJohannes Gutenberg University Medical CenterMainz Germany
  3. 3.Ganymed PharmaceuticalsMainzGermany
  4. 4.TRON-Translational Oncology at the University Medical Center MainzMainzGermany
  5. 5.III. Medical DepartmentUniversity Medical Center of the Johannes Gutenberg-University MainzMainzGermany
  6. 6.Ribological, Biontech AGMainzGermany

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