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Synthetic Messenger RNA and Cell Metabolism Modulation pp 235–246Cite as

Antitumor Vaccination with Synthetic mRNA: Strategies for In Vitro and In Vivo Preclinical Studies

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 969))

Abstract

Synthetic antigen-encoding mRNA is increasingly exploited as a tool for delivery of genetic information of complete antigens into professional antigen presenting dendritic cells for HLA haplotype-independent antigen-specific vaccination against cancer. Two strategies for mRNA-based antitumor vaccination have emerged into the clinical setting. One is transfection of autologous dendritic cells with synthetic mRNA for adoptive transfer into the patient. The other is direct injection of naked synthetic mRNA. Both methods have proven to be feasible and safe and to elicit antigen-specific immune responses. The design of novel synthetic vaccines employing synthetic mRNA requires further in-depth investigation of its bioavailability and immune pharmacology. This chapter summarizes the state-of-art in this field and describes methods elementary for preclinical studies of mRNA-based antitumor vaccine protocols.

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Acknowledgments

This research was supported by the GO-Bio funding of the German Federal Ministry of Education and Research to Ugur Sahin.

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Authors and Affiliations

  1. Translational Oncology and Immunology (TRON) at the Johannes Gutenberg University Mainz, Mainz, Germany

    Mustafa Diken, Sebastian Kreiter & Abderraouf Selmi

  2. Division of Translational and Experimental Oncology, Department of Internal Medicine II, Johannes Gutenberg University Medical Center, Mainz , Germany

    Abderraouf Selmi

  3. Ganymed Pharmaceuticals, Mainz, Germany

    Özlem Türeci

  4. TRON-Translational Oncology at the University Medical Center Mainz, Mainz, Germany

    Ugur Sahin

  5. III. Medical Department, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany

    Ugur Sahin

  6. Ribological, Biontech AG, Mainz, Germany

    Ugur Sahin

Authors
  1. Mustafa Diken
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  2. Sebastian Kreiter
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  3. Abderraouf Selmi
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  4. Özlem Türeci
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  5. Ugur Sahin
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Corresponding author

Correspondence to Ugur Sahin .

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Editors and Affiliations

  1. The Anlyan Center, Department of Genetics, Yale School of Medicine, Cedar Street 300, New Haven, 06520-8005, Connecticut, USA

    Peter M. Rabinovich

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Diken, M., Kreiter, S., Selmi, A., Türeci, Ö., Sahin, U. (2013). Antitumor Vaccination with Synthetic mRNA: Strategies for In Vitro and In Vivo Preclinical Studies. In: Rabinovich, P. (eds) Synthetic Messenger RNA and Cell Metabolism Modulation. Methods in Molecular Biology, vol 969. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-260-5_15

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  • DOI: https://doi.org/10.1007/978-1-62703-260-5_15

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-259-9

  • Online ISBN: 978-1-62703-260-5

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