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Fungal Diagnostics

Volume 968 of the series Methods in Molecular Biology pp 141-147

Date:

Applied Gene Histopathology: Identification of Fusarium Species in FFPE Tissue Sections by In Situ Hybridization

  • Yoichiro OkuboAffiliated withDepartment of Surgical Pathology, Toho University School of Medicine
  • , Minoru ShinozakiAffiliated withDepartment of Surgical Pathology, Toho University School of Medicine
  • , Megumi WakayamaAffiliated withDepartment of Surgical Pathology, Toho University School of Medicine
  • , Haruo NakayamaAffiliated withDepartment of Neurosurgery, Toho University Ohashi Medical Center
  • , Daisuke SasaiAffiliated withDepartment of Surgical Pathology, Toho University School of Medicine
  • , Takao IshiwatariAffiliated withDepartment of Surgical Pathology, Toho University School of Medicine
  • , Tetsuo NemotoAffiliated withDepartment of Surgical Pathology, Toho University School of Medicine
  • , Tochigi NaobumiAffiliated withDepartment of Surgical Pathology, Toho University School of Medicine
  • , Kazutoshi ShibuyaAffiliated withDepartment of Surgical Pathology, Toho University School of Medicine Email author 

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Abstract

Although accurate and rapid diagnosis of fusariosis is now required, morphological similarities among molds make it difficult to histologically differentiate Fusarium spp. from other molds. In this chapter, we present our in situ hybridization (ISH) technique as a valuable tool to identify Fusarium spp. and emphasize the usefulness of the technique.

Key words

In situ hybridization Fusarium species Invasive fungal infection Immunocompetent individual Peptide nucleic acid Formalin-fixed and paraffin-embedded tissue section 28S rRNA