Subfractionation and Analysis of the Cell Envelope (Lipo)polysaccharides of Mycobacterium tuberculosis

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 966)

Abstract

The cell envelope ofMycobacterium tuberculosis, the causative agent of tuberculosis in humans, is the source of carbohydrates of exceptional structure which play essential roles in the physiology of the bacterium and in its interactions with the host during infection. Much of what is known about their biosynthesis was derived from the phenotypic analysis of knockout or conditional knockout mutants of mycobacteria generated by random or specific insertional mutagenesis. Here, we describe the current techniques used to subfractionateM. tuberculosiscells and investigate major quantitative and qualitative changes in their cell envelope (lipo)polysaccharides.

Key words

Mycobacterium tuberculosis Arabinogalactan Lipoarabinomannan Lipomannan Glucan Capsule 

Notes

Acknowledgments

Research on mycobacterial (lipo)polysaccharides in the authors’ laboratory is supported by the National Institutes of Health/National Institute of Allergy and Infectious Diseases grant AI064798. Dr. Shiva Kumar Angala is gratefully acknowledged for his assistance in the preparation of Fig.3.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Mycobacteria Research Laboratories, Department of Microbiology, Immunology, and PathologyColorado State UniversityFort CollinsUSA

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