Cell Senescence pp 373-382

Part of the Methods in Molecular Biology book series (MIMB, volume 965)

Genome-Wide RNAi Screening to Identify Regulators of Oncogene-Induced Cellular Senescence

  • Narendra Wajapeyee
  • Sara K. Deibler
  • Michael R. Green
Protocol

Abstract

RNA interference (RNAi) is a powerful research tool that can be used to turn off—or silence—the expression of a specific gene. In recent years, RNAi screening on a genome-wide scale has provided the opportunity to identify factors and pathways involved in complex biological processes in a systematic and unbiased manner. Here we describe a genome-wide RNAi screening strategy to identify genes that are required for a particular type of cellular senescence called oncogene-induced senescence. The approach we describe is a general screening strategy that can be applied to the study of other forms of cellular senescence, including replicative senescence.

Key words

Functional genomics Genome-wide Oncogene-induced senescence Positive-selection screen RNA interference siRNA shRNA 

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Copyright information

© Springer Science+Busincess Media, LLC 2013

Authors and Affiliations

  • Narendra Wajapeyee
    • 1
  • Sara K. Deibler
    • 2
    • 3
  • Michael R. Green
    • 2
    • 3
  1. 1.Department of PathologyYale University School of MedicineNew HavenUSA
  2. 2.Howard Hughes Medical InstituteChevy ChaseUSA
  3. 3.Programs in Gene Function and Expression and Molecular MedicineUniversity of Massachusetts Medical SchoolWorcesterUSA

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