Profiling the Metabolic Signature of Senescence

  • Florian M. Geier
  • Silke Fuchs
  • Gabriel Valbuena
  • Armand M. Leroi
  • Jacob G. BundyEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 965)


Aging is a complex process, which involves changes in different cellular functions that all can be integrated on the metabolite level. This means that different gene regulation pathways that affect aging might lead to similar changes in metabolism and result in a metabolic signature of senescence. In this chapter, we describe how to establish a metabolic signature of senescence by analyzing the metabolome of various longevity mutants of the model organism Caenorhabditis elegans using gas chromatography-mass spectrometry (GC-MS). Since longevity-associated genes exist for other model organisms and humans, this analysis could be universally applied to body fluids or whole tissue samples for studing the relationship between senescence and metabolism.

Key words

Aging Metabolomics Caenorhabditis elegans Cellular senescence GC-MS 



Manuel Liebeke is thanked for the fruitful discussions of the manuscript. FMG acknowledges the AXA Research Fund for funding.


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Copyright information

© Springer Science+Busincess Media, LLC 2013

Authors and Affiliations

  • Florian M. Geier
    • 1
  • Silke Fuchs
    • 2
  • Gabriel Valbuena
    • 1
  • Armand M. Leroi
    • 3
  • Jacob G. Bundy
    • 1
    Email author
  1. 1.Biomolecular Medicine, Department of Surgery and CancerImperial CollegeLondonUK
  2. 2.Cell and Molecular Biology, Department of Life SciencesImperial CollegeLondonUK
  3. 3.Ecology and Evolution, Department of Life SciencesImperial CollegeLondonUK

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