p53 Protocols pp 165-181 | Cite as

p53 Actions on MicroRNA Expression and Maturation Pathway

  • Hiroshi I. Suzuki
  • Kohei MiyazonoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 962)


The tumor suppressor p53 orchestrates multiple cellular pathways as a central node of anti-oncogenic programs in response to DNA damage, oncogene activation, and several stresses. In addition to the principal role as a transcription factor that transactivates many target genes involved in apoptosis and cell cycle control, p53 has been shown to exert various transactivation-independent effects both in the nucleus and in the cytoplasm. Diversity of p53 activities is further emphasized by the recent studies revealing the close interaction between the p53 and microRNA (miRNA) world. We recently demonstrated that p53 promotes the processing of several primary miRNA transcripts through association with Drosha, a central RNase III in miRNA biogenesis, under DNA damage-inducing conditions. In contrast to wild-type p53, cancer-derived p53 mutants attenuate miRNA maturation. These findings reveal a novel aspect of p53 activities and suggest complex crosstalks between miRNA biogenesis and intracellular signaling pathways. In this chapter, we describe the methods for evaluation of the effects of p53 on miRNA expression, an interaction between pri-miRNA and Drosha complex, and pri-miRNA processing activity of the Drosha complex.

Key words

microRNA p53 p68 Drosha Dicer Processing 



We thank K. Yamagata and S. Kato for technical advice on in vitro processing analysis. This work was supported by KAKENHI (Grant-in-Aid for Scientific Research) and the Global Center of Excellence Program for “Integrative Life Science Based on the Study of Biosignaling Mechanisms” from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. H.I.S. is supported by a research fellowship of the Japan Society for the Promotion of Science for Young Scientists.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Department of Molecular Pathology, Graduate School of MedicineUniversity of TokyoTokyoJapan

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