RNAi-Mediated Gene Function Analysis in Skin

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 961)

Abstract

We have recently developed a method for RNAi-mediated gene function analysis in skin (Beronja et al., Nat Med 16:821–827, 2010). It employs ultrasound-guided in utero microinjections of lentivirus into the amniotic cavity of embryonic day 9 mice, which result in rapid, efficient, and stable transduction into mouse skin. Our technique greatly extends the available molecular and genetic toolbox for comprehensive functional examination of outstanding problems in epidermal biology. In its simplest form, as a single-gene function analysis via shRNA-mediated gene knockdown, our technique requires no animal mating and may need as little as only a few days between manipulation and phenotypic analysis.

Key words

Lentivirus Intraamniotic In utero In vivo RNAi shRNA RNA interference Ultrasound-guided microinjection 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Laboratory of Mammalian Cell Biology & Development, Howard Hughes Medical InstituteThe Rockefeller UniversityNew YorkUSA

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