Plant Organogenesis pp 265-275

Part of the Methods in Molecular Biology book series (MIMB, volume 959) | Cite as

Regeneration in Arabidopsis Tissue Culture

Protocol

Abstract

An entire Arabidopsis plant can be regenerated from a small piece of tissue by two sequential hormonal treatments in tissue culture. Currently this in vitro regeneration system is a good system to study the mechanism by which plants show regenerative plasticity. Also, it is useful to test the hormone sensitivity of plants and to propagate sterile lines in Arabidopsis. Here we describe a standard protocol for regenerating Arabidopsis plants in tissue culture, and for preparing and observing samples using confocal microscopy to study cells during regeneration.

Key words

Arabidopsis thaliana Plant regeneration Tissue culture Plant hormone Callus formation Shoot induction Root induction 

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Division of Biology 156-29California Institute of TechnologyPasadenaUSA

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