Plant Organogenesis pp 265-275
Regeneration in Arabidopsis Tissue Culture
An entire Arabidopsis plant can be regenerated from a small piece of tissue by two sequential hormonal treatments in tissue culture. Currently this in vitro regeneration system is a good system to study the mechanism by which plants show regenerative plasticity. Also, it is useful to test the hormone sensitivity of plants and to propagate sterile lines in Arabidopsis. Here we describe a standard protocol for regenerating Arabidopsis plants in tissue culture, and for preparing and observing samples using confocal microscopy to study cells during regeneration.
Key wordsArabidopsis thaliana Plant regeneration Tissue culture Plant hormone Callus formation Shoot induction Root induction
- 1.Skoog F, Miller CO (1957) Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp Soc Exp Biol 54:118–130Google Scholar
- 7.Bechtold N, Ellis J, Pelletier G (1993) In planta Agrobacterium- mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. C R Acad Sci Paris, Life Sci 316: 1194–1199Google Scholar
- 9.Weigel D, Glazebrook J (2002) Arabidopsis: a laboratory manual. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
- 13.Akama K, Puchta H, Hohn B (1995) Efficient Agrobacterium-mediated transformation of Arabidopsis thaliana using the bar gene as selectable marker. Pant Cell Rep 14:450–454Google Scholar