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Assembly of Matched Alpha/Beta Subunits to HLA Class II Peptide Receptors

  • Sebastian TemmeEmail author
  • Nadine Kämper
  • Norbert Koch
Protocol
Part of the Methods in Molecular Biology™ book series (MIMB, volume 960)

Abstract

Human antigen presenting cells express three human leukocyte antigen (HLA) class II isotypes (DR, DP, and DQ), which are composed of polymorphic α and β subunits. The combination of polymorphic α- and β-chains results in cis (encoded on the same chromosome) or trans (encoded on different chromosomes) combinations. Since some of the α–β combinations may yield mismatched non-functional α–β heterodimers, it is not entirely clear which type of HLA class II peptide receptors are found on the cell surface of antigen presenting cells. We have developed a combination of biochemical techniques for inspection of the assembly and intracellular transport of isotype matched and mismatched class II heterodimers.

Key words

MHC class II molecules Invariant chain Intracellular transport Endoglycosidase H SDS-stable MHCII dimers Flow cytometry 

Notes

Acknowledgements

We thank Monica Pohle for critical reading of the manuscript and Angelika König for expert technical assistance This work was supported by a grant from the Deutsche Forschungsgemeinschaft.

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Sebastian Temme
    • 1
    • 2
    Email author
  • Nadine Kämper
    • 2
  • Norbert Koch
    • 2
  1. 1.Institute of Molecular CardiologyUniversity of DüsseldorfDüsseldorfGermany
  2. 2.Division of Immunobiology, Institute of GeneticsUniversity of BonnBonnGermany

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