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Functional Characterization of Rice Genes Using a Gene-Indexed T-DNA Insertional Mutant Population

  • Ki-Hong Jung
  • Gynheung AnEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 956)

Abstract

Despite the availability of the finished genome sequence and tools for its analyses, few rice genes have been characterized. Because Agrobacterium-mediated transformation causes random T-DNA insertions across the genome, T-DNA can be a good mutagen for functional genomics. Gene-indexed mutants with flanking sequences around inserted T-DNA are valuable resources for accelerating functional characterizations of rice genes. Such mutant lines, covering at least half the rice genome, have been generated through international efforts. Here, we describe approaches that use functional genomics with T-DNA insertional mutagenesis.

Key words

Activation tagging Gene-indexed Insertional mutagenesis Reporter Rice T-DNA 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Department of Plant PathologyUniversity of CaliforniaDavisUSA
  2. 2.Department of Plant Molecular Systems Biotechnology, Crop Biotech InstituteKyung Hee UniversityYonginRepublic of Korea
  3. 3.Department of Plant Molecular Systems BiotechnologyKyung Hee UniversityYonginRepublic of Korea
  4. 4.Crop Biotech InstituteKyung Hee UniversityYonginRepublic of Korea

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