Mammalian Oocyte Regulation

Volume 957 of the series Methods in Molecular Biology pp 267-283


Microarray-CGH for the Assessment of Aneuploidy in Human Polar Bodies and Oocytes

  • Souraya JaroudiAffiliated withReprogenetics UK, Institute of Reproductive Sciences
  • , Dagan WellsAffiliated withNuffield Department of Obstetrics and Gynaecology, Women’s Centre John Radcliffe Hospital, University of Oxford Email author 

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The cytogenetic analysis of single cells, such as oocytes and polar bodies, is extremely challenging. The main problem is low probability of obtaining a metaphase preparation in which all of the chromosomes are sufficiently well spread to permit accurate analysis (no overlapping chromosomes, no chromosomes lost). As a result, a high proportion of the oocytes subjected to cytogenetic analysis are not suitable for traditional chromosome banding studies or for molecular cytogenetic methods such as spectral karyotyping (SKY) or multiplex fluorescence in situ hybridization (M-FISH). Fortunately, recent innovations in whole genome amplification and microarray technologies have provided a means to analyze the copy number of every chromosome in single cells with high accuracy. Here we describe the use of such methods for the investigation of chromosome and chromatid abnormalities in human oocytes and polar bodies.

Key words

Whole genome amplification Microarray Comparative genomic hybridization Aneuploidy Polar body Oocyte