High-Resolution Imaging of 2D Outer Membrane Protein F Crystals by Atomic Force Microscopy

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 955)

Abstract

In this chapter the methodological bases are provided to achieve subnanometer resolution on two-dimensional (2D) membrane protein crystals by atomic force microscopy (AFM). This is outlined in detail with the example of AFM studies of the outer membrane protein F (OmpF) from the bacterium Escherichia coli (E. coli). We describe in detail the high-resolution imaging of 2D OmpF crystals in aqueous solution and under near-physiological conditions. The topographs of OmpF, and stylus effects and artifacts encountered when imaging by AFM are discussed.

Key words

Atomic force microscopy Biological membranes High resolution Outer membrane protein F Stylus artifacts Topography 

Notes

Acknowledgments

We are grateful to Andreas Engel for his continous support. This work was supported by the Swiss National Foundation for Scientific Research (Grant 31003A_125150 to D.F.), the National Center of Competence in Research (NCCR) TransCure, and the European Science Foundation (Grant 09-EuroSYNBIO-FP-012 NANOCELL to D.F. and D.J.M.).

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Institute of Biochemistry and Molecular MedicineUniversity of BernBernSwitzerland
  2. 2.ETH Zürich, Biosystems Science and Engineering (BSSE)BaselSwitzerland

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