Analyzing PPARα/Ligand Interactions by Chemical Cross-Linking and High-Resolution Mass Spectrometry

  • Mathias Q. Müller
  • Christian H. Ihling
  • Andrea Sinz
Part of the Methods in Molecular Biology book series (MIMB, volume 952)


The combination of chemical cross-linking and high-resolution mass spectrometry is an emerging technique for monitoring conformational changes in proteins induced by drug binding. In this chapter, we describe this approach for gaining insights into the conformational changes of the peroxisome proliferator-activated receptor alpha after binding of low-molecular weight ligands. Our strategy provides a basis to efficiently characterize target protein-drug interactions.

Key words

PPARα/ligand interaction Chemical cross-linking Isotope labeling SDS-PAGE In-gel digestion Nano-HPLC High-resolution mass spectrometry 


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Mathias Q. Müller
    • 1
  • Christian H. Ihling
    • 1
  • Andrea Sinz
    • 1
  1. 1.Department of Pharmaceutical Chemistry and BioanalyticsInstitute of Pharmacy, Martin-Luther-Universität Halle-WittenbergHalle (Saale)Germany

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