Plant Cell Suspension Cultures

  • Roberto Moscatiello
  • Barbara Baldan
  • Lorella Navazio
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 953)

Abstract

Plant cell suspension cultures are widely used in plant biology as a convenient tool for the investigation of a wide range of phenomena, bypassing the structural complexity of the plant organism in toto. The homogeneity of an in vitro cell population, the large availability of material, the high rate of cell growth and the good reproducibility of conditions make suspension-cultured cells suitable for the analysis of complex physiological processes at the cellular and molecular levels. Moreover, plant cell cultures provide a valuable platform for the production of high-value secondary metabolites and other substances of commercial interest. Here we describe how to initiate and maintain plant cell cultures starting from explants obtained from in vitro germinated seedlings. Isolation of protoplasts from plant cell suspension cultures and regeneration of plants via organogenesis and somatic embryogenesis are also presented.

Key words

Plant explants Culture medium Plant hormones Callus Cell suspension Protoplasts Organogenesis Somatic embryogenesis 

Notes

Acknowledgements

This work was supported by Programmi di Ricerca Scientifica di Rilevante Interesse Nazionale (2008WKPAWW to LN) and Ricerca Scientifica quota EX-60% (60A06-2248/11 to LN and 60A06-2448/11 to BB).

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Roberto Moscatiello
    • 1
  • Barbara Baldan
    • 1
  • Lorella Navazio
    • 2
  1. 1.Department of BiologyUniversity of PadovaPadovaItaly
  2. 2.Dipartimento di BiologiaUniversità di PadovaPadovaItaly

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