Plant Mineral Nutrients pp 171-183

Part of the Methods in Molecular Biology book series (MIMB, volume 953) | Cite as

Ion Flux Measurements Using the MIFE Technique

  • Sergey Shabala
  • Lana Shabala
  • Jayakumar Bose
  • Tracey Cuin
  • Ian Newman
Protocol

Abstract

Noninvasive microelectrode ion flux measurements (the MIFE™ technique) allow the concurrent quantification of net fluxes of several ions with high spatial (several μm) and temporal (ca 5 s) resolution. The MIFE technique has become a popular tool for studying the adaptive responses of plant cells and tissues to a large number of abiotic and biotic stresses. This chapter briefly summarizes some key findings on spatial and temporal organization of plant nutrient acquisition obtained by the MIFE technique, as well as the MIFE contribution towards elucidating the mechanisms behind a plant’s perception and signaling of major abiotic stresses. The full protocols for microelectrode fabrication, calibration, and use are then given, and two basic routines for mapping root ion flux profiles and studying transient ion flux kinetics are given.

Key words

Ion flux Membrane transport Ion-selective microelectrodes Deficiency Toxicity Acidity Salinity Oxidative stress 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Sergey Shabala
    • 1
  • Lana Shabala
    • 1
  • Jayakumar Bose
    • 2
  • Tracey Cuin
    • 2
    • 3
  • Ian Newman
    • 4
  1. 1.School of Agricultural ScienceUniversity of TasmaniaHobartAustralia
  2. 2.School of Agricultural ScienceUniversity of TasmaniaHobartAustralia
  3. 3.Biochimie et Physiologie Moléculaire des Plantes, Institut National de la Recherche Agronomique (INRA), Montpellier SupAgroUniversité Montpellier IIMontpellierFrance
  4. 4.School of Mathematics and PhysicsUniversity of TasmaniaHobartAustralia

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