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Synthesis of Polyethylenimine-Based Nanocarriers for Systemic Tumor Targeting of Nucleic Acids

  • Wolfgang Rödl
  • David Schaffert
  • Ernst Wagner
  • Manfred OgrisEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 948)

Abstract

Nucleic acid-based therapies offer the option to treat tumors in a highly selective way, while toxicity towards healthy tissue can be avoided when proper delivery vehicles are used. We have recently developed carrier systems based on linear polyethylenimine, which after chemical coupling of proteinous or peptidic ligands can form nanosized polyplexes with plasmid DNA or RNA and deliver their payload into target cells by receptor-mediated endocytosis. This chapter describes the synthesis of linear PEI (LPEI) from a precursor polymer and the current coupling techniques and purification procedure for peptide conjugates with linear polyethylenimine. A protocol is also given for the formation and characterization of polyplexes formed with LPEI conjugate and plasmid DNA.

Key words

Polyethylenimine Polyethylene glycol Molecular conjugates EGF receptor Targeting Gene delivery 

Notes

Acknowledgments

This work was supported by the Center for Nanoscience (CeNS) and the German Research Foundation (SFB824) to M.O., and the Nanosystems Initiative Munich (NIM) to E.W.

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Wolfgang Rödl
    • 1
  • David Schaffert
    • 2
    • 3
  • Ernst Wagner
    • 2
    • 4
  • Manfred Ogris
    • 5
    • 4
    Email author
  1. 1.Department of Pharmacy, Center for System Based Drug Research, Pharmaceutical BiotechnologyLudwig-Maximilians-UniversityMunichGermany
  2. 2.Department of Pharmacy, Center for System based Drug Research, Pharmaceutical BiotechnologyLudwig-Maximilians-UniversityMunichGermany
  3. 3.Department of Molecular BiologyAarhus UniversityAarhus CDenmark
  4. 4.Center for NanoScience (CeNS)Ludwig-Maximilians-UniversityMunichGermany
  5. 5.Center for System based Drug Research, Department of Pharmacy, Pharmaceutical BiotechnologyLudwig-Maximilians-UniversityMunichGermany

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