In Situ AFM Analysis Investigating Disassembly of DNA Nanoparticles and Nano-Films

  • Yi Zou
  • Lei Wan
  • Jenifer Blacklock
  • David Oupicky
  • Guangzhao Mao
Part of the Methods in Molecular Biology book series (MIMB, volume 948)


Synthetic vector-based gene delivery systems continue to gain strength as viable alternatives to viral vectors due to safety and other concerns. DNA release dynamics is key to the understanding and control of gene delivery from nano-systems. Here we describe atomic force microscope application to the understanding of DNA release dynamics from bioreducible polycation-based nano-systems. The two nano-systems are polyplex nanoparticles and layer-by-layer films.

Key words

In situ AFM Gene delivery DNA release Polyplex Layer-by-layer film 


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Yi Zou
    • 1
  • Lei Wan
    • 1
  • Jenifer Blacklock
    • 2
  • David Oupicky
    • 3
  • Guangzhao Mao
    • 3
  1. 1.Department of Chemical Engineering and Material SciencesWayne State UniversityDetroitUSA
  2. 2.Materials Reliability DivisionNational Institute of Standards and TechnologyBoulderUSA
  3. 3.Department of Pharmaceutical SciencesWayne State UniversityDetroitUSA

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