Microfluidic Diagnostics pp 387-401

Part of the Methods in Molecular Biology book series (MIMB, volume 949) | Cite as

Multilayer Microfluidic Poly(Ethylene Glycol) Diacrylate Hydrogels



Development of robust, in vivo like tissues in vitro holds the potential to create regenerative medicine-based therapeutics, provide more physiologically significant preclinical models and supply a pharmacological and toxicological screening platform that reflects in vivo systems in both complexity and function. This protocol describes a simple, robust, multilayer replica molding technique in which poly(dimethylsiloxane) (PDMS) and poly(ethylene glycol) diacrylate (PEGDA) are serially replica molded to develop microfluidic PEGDA hydrogel networks embedded within independently fabricated PDMS housings, using a combination of soft and photo-lithography. This work has direct applications toward the development of robust, complex, cell-laden hydrogels for in vitro diagnostics and regenerative medicine applications.

Key words

Hydrogel Poly (ethylene glycol) Microfabrication Microfluidics Photolithography Soft lithography 


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Copyright information

© Springer Science+Business Media,LLC 2013

Authors and Affiliations

  1. 1.Department of BioengineeringMS-142, BRC, Rice UniversityHoustonUSA

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