Zebrafish Teratogenicity Testing

  • Kimberly C. BrannenEmail author
  • Jeffrey H. Charlap
  • Elise M. Lewis
Part of the Methods in Molecular Biology book series (MIMB, volume 947)


As a model for teratogenicity research, zebrafish are gaining popularity and creditability. Zebrafish embryos have been proven to be a highly valuable tool in genetics and developmental biology research and have advanced our understanding of a number of known developmental toxicants. It has yet to be determined conclusively how reliably a zebrafish embryo screening assay predicts what will happen in mammalian models, but results from initial assessments have been encouraging. Here we have presented procedures for the basic care of a zebrafish colony to support embryo production, embryo collection and culturing, and teratogenicity experiments.

Key words

Zebrafish Teratogenicity Alternative methods 


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Kimberly C. Brannen
    • 1
    Email author
  • Jeffrey H. Charlap
    • 2
  • Elise M. Lewis
    • 1
  1. 1.Charles River LaboratoriesHorshamUSA
  2. 2.WIL Research LaboratoriesAshlandUSA

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