Isolation and Culture of Alveolar Epithelial Type I and Type II Cells from Rat Lungs

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 945)

Abstract

The pulmonary alveolar epithelium, comprised of alveolar Type I (TI) and Type II (TII) cells, covers more than 99% of the internal surface area of the lungs. The study of isolated and cultured alveolar epithelial TI and TII cells has provided a large amount of information about the functions of both cell types. This chapter provides information about methods for isolating and culturing both of these cell types from rat lungs.

Key words

Lung Alveolar epithelium Type I cells Type II cells 

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.The Cardiovascular Research InstituteUniversity of CaliforniaSan FranciscoUSA
  2. 2.Departments of Medicine and Pediatrics, The Cardiovascular Research InstituteUniversity of CaliforniaSan FranciscoUSA

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